Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley

@article{imkov2008CouplingAD,
  title={Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley},
  author={Hana {\vS}imkov{\'a} and Jan T. Svensson and Pascal Condamine and Eva Hřibov{\'a} and Pavla Such{\'a}nkov{\'a} and Prasanna Bhat and Jan Barto{\vs} and Jan {\vS}af{\'a}ř and Timothy J. Close and Jaroslav Dole{\vz}el},
  journal={BMC Genomics},
  year={2008},
  volume={9},
  pages={294 - 294}
}
Flow cytometry facilitates sorting of single chromosomes and chromosome arms which can be used for targeted genome analysis. However, the recovery of microgram amounts of DNA needed for some assays requires sorting of millions of chromosomes which is laborious and time consuming. Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome… CONTINUE READING
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