Coupled reverse transcription-polymerase chain reaction (RT-PCR) technique is comparative, quantitative, and rapid: uses in alcohol research involving low abundance mRNA species such as hypothalamic LHRH and GRF.

@article{Kelley1993CoupledRT,
  title={Coupled reverse transcription-polymerase chain reaction (RT-PCR) technique is comparative, quantitative, and rapid: uses in alcohol research involving low abundance mRNA species such as hypothalamic LHRH and GRF.},
  author={Mark R Kelley and Jan Jurgens and John Tentler and Nicholas V. Emanuele and Sarah E. Blutt and Mary Ann Emanuele},
  journal={Alcohol},
  year={1993},
  volume={10 3},
  pages={
          185-9
        }
}
The measurement of alterations in low abundance mRNAs such as the hypothalamic hormones luteinizing hormone-releasing hormone (LHRH) and growth hormone-releasing hormone (GHRH or GRF) from individual hypothalamic tissues in rats has previously been difficult and usually required either isolation of poly(A) mRNA or the pooling of numerous animals to obtain a reasonable signal on Northern blots. Although more sensitive detection methods exist, such as the use of RNA probes or solution… CONTINUE READING

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