Coupled bioconversion for preparation of N-acetyl-d-neuraminic acid using immobilized N-acetyl-d-glucosamine-2-epimerase and N-acetyl-d-neuraminic acid lyase

@article{Hu2009CoupledBF,
  title={Coupled bioconversion for preparation of N-acetyl-d-neuraminic acid using immobilized N-acetyl-d-glucosamine-2-epimerase and N-acetyl-d-neuraminic acid lyase},
  author={Shiyuan Hu and Jun Chen and Zhong-Yi Yang and Lijun Shao and Hua Bai and Jiali Luo and Wei-hong Jiang and Yunliu Yang},
  journal={Applied Microbiology and Biotechnology},
  year={2009},
  volume={85},
  pages={1383-1391}
}
N-Acetyl-d-neuraminic acid (Neu5Ac) can be produced from N-acetyl-d-glucosamine (GlcNAc) and pyruvate by a chemoenzymatic process in which an alkaline-catalyzed epimerization transforms GlcNAc to N-acetyl-d-manosamine (ManNAc). ManNAc is then condensed biocatalytically with pyruvate in the presence of N-acetyl-d-neuraminic acid lyase (NAL) or by a two-step, fully enzymatic process involving bioconversions of GlcNAc to ManNAc and ManNAc to Neu5Ac using N-acetyl-d-glucosamine 2-epimerase (AGE… CONTINUE READING
10 Citations
25 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 25 references

Characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid (Neu5Ac)

  • S Blayer, JM Woodley, MD Lilly, MJ Dawson
  • Biotechnol Prog
  • 1996
Highly Influential
12 Excerpts

nacetylneuraminic acid from N-acetylglucosamine and pyruvate using recombinant human rennin binding protein and sialic acid aldolase in one pot

  • JO Lee, JK Yi, SG Lee, S Takahashi, BG Kim
  • Enzyme Microb Technol
  • 2003
Highly Influential
1 Excerpt

Production of Nacetyl-D-neuraminic acid by coupling bacteria expressing Nacetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid synthetase

  • K Tabata, S Koizumi, T Endo, A Ozaki
  • Enzyme Microb Technol
  • 2002
Highly Influential
5 Excerpts

Enzymatic twostep synthesis of N-acetyl-neuraminic acid in the enzyme membrane reactor

  • U Kragle, D Gygax, O Ghisalba, C Wandrey
  • Angew Chem Int Ed Engl
  • 1991
Highly Influential
4 Excerpts

Enzymes in carbohydrate synthesis: N-acetyl-D-neuraminic acid aldolasecatalyzed reactions and preparations of N-acetyl-2-deoxy-Dneuraminic acid derivaties

  • MJ Kim, WJJ Hennen, SM Sweers, CH Wong
  • J Am Chem Soc 110:6464–6484
  • 1987
Highly Influential
2 Excerpts

The sialic acids. The structure and enzymatic synthesis of N-acetyl-D-neuraminic acid

  • DG Roseman
  • J Biol Chem
  • 1960
Highly Influential
2 Excerpts

Similar Papers

Loading similar papers…