Coupled assay of sphingomyelin and ceramide molecular species by gas liquid chromatography.

@article{Vieu2002CoupledAO,
  title={Coupled assay of sphingomyelin and ceramide molecular species by gas liquid chromatography.},
  author={Claude Vieu and François Terc{\'e} and Françoise Chevy and Corinne Rolland and Ronald Barbaras and Hugues Chap and Claude Wolf and Bertrand Perret and Xavier Collet},
  journal={Journal of lipid research},
  year={2002},
  volume={43 3},
  pages={510-22}
}
This study reports a single-step analysis of the molecular species of endogenous ceramides and of the ceramide moiety of sphingomyelins in biological samples, using gas liquid chromatography (GLC). Silylated sphingomyelins were quantitatively converted to monosilylated ceramide upon injection into GLC, whereas the free ceramides were di-silylated on the primary and secondary alcohol function, as confirmed by mass spectrometry. The reproducible shift of the retention times between the mono- and… CONTINUE READING

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