Counting nucleosomes in living cells with a combination of fluorescence correlation spectroscopy and confocal imaging.

@article{Weidemann2003CountingNI,
  title={Counting nucleosomes in living cells with a combination of fluorescence correlation spectroscopy and confocal imaging.},
  author={Thomas Weidemann and Malte Wachsmuth and Tobias A. Knoch and Gabriele Mueller and Waldemar Waldeck and J{\"o}rg Langowski},
  journal={Journal of molecular biology},
  year={2003},
  volume={334 2},
  pages={229-40}
}
Although methods for light microscopy of chromatin are well established, there are no quantitative data for nucleosome concentrations in vivo. To establish such a method we used a HeLa clone expressing the core histone H2B fused to the enhanced yellow fluorescent protein (H2B-EYFP). Quantitative gel electrophoresis and fluorescence correlation spectroscopy (FCS) of isolated oligonucleosomes show that 5% of the total H2Bs carry the fluorescent tag and an increased nucleosome repeat length of 204… CONTINUE READING

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