Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR.

@article{Suzuki2012CounterselectionSF,
  title={Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR.},
  author={Hirokazu Suzuki and Ayano Murakami and Ken-ichi Yoshida},
  journal={Applied and environmental microbiology},
  year={2012},
  volume={78 20},
  pages={7376-83}
}
Counterselection systems facilitate marker-free genetic modifications in microbes by enabling positive selections for both the introduction of a marker gene into the microbe and elimination of the marker from the microbe. Here we report a counterselection system for Geobacillus kaustophilus HTA426, established through simultaneous disruption of the pyrF and… CONTINUE READING

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