Counterflow centrifugation allows addition of appropriate numbers of T cells to allogeneic marrow and blood stem cell grafts to prevent severe GVHD without substantial loss of mature and immature progenitor cells

Abstract

Using counterflow centrifugation elutriation (CCE) lymphocytes can be separated from CD34+ populations based on size. Immature progenitors tend to be smaller than mature cells suggesting that CCE introduces loss of stem cells. We compared the separation of 12 PBSC with 16 BM transplants. Cells were separated in 12 fractions (3000–2200 r.p.m.) and the rotor… (More)
DOI: 10.1038/sj.bmt.1701775

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@article{Preijers1999CounterflowCA, title={Counterflow centrifugation allows addition of appropriate numbers of T cells to allogeneic marrow and blood stem cell grafts to prevent severe GVHD without substantial loss of mature and immature progenitor cells}, author={F W M B Preijers and PB van Hennik and A V M B Schattenberg and Petrus A. Ruijs and R. E. Ploemacher and T. M. C. de Witte}, journal={Bone Marrow Transplantation}, year={1999}, volume={23}, pages={1061-1070} }