Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis.

@article{Quast2015CotranslationalIO,
  title={Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis.},
  author={Robert B Quast and Devid Mrusek and Christian M. Hoffmeister and Andrei Sonnabend and Stefan Kubick},
  journal={FEBS letters},
  year={2015},
  volume={589 15},
  pages={
          1703-12
        }
}
Over the last years protein engineering using non-standard amino acids has gained increasing attention. As a result, improved methods are now available, enabling the efficient and directed cotranslational incorporation of various non-standard amino acids to equip proteins with desired characteristics. In this context, the utilization of cell-free protein synthesis is particularly useful due to the direct accessibility of the translational machinery and synthesized proteins without having to… CONTINUE READING

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