Cotranslational folding of globin.

  title={Cotranslational folding of globin.},
  author={Anton A. Komar and A. van den Kommer and I. Krasheninnikov and Alexander S. Spirin},
  journal={The Journal of biological chemistry},
  volume={272 16},
Globin synthesis in a wheat germ cell-free translation system was performed in the presence of [3H]hemin and [35S]methionine to determine the minimal length of the nascent ribosome-bound globin chain capable of heme binding. Nascent polypeptides of predetermined size were synthesized on ribosomes by translation of truncated mRNA molecules. Analysis with the use of sucrose gradient centrifugation and puromycin reaction revealed that the ribosome-bound N-terminal alpha-globin fragments of 140… CONTINUE READING