Cosuppression in Drosophila: Gene Silencing of Alcohol dehydrogenase by white-Adh Transgenes Is Polycomb Dependent

@article{PalBhadra1997CosuppressionID,
  title={Cosuppression in Drosophila: Gene Silencing of Alcohol dehydrogenase by white-Adh Transgenes Is Polycomb Dependent},
  author={Manika Pal‐Bhadra and Utpal Bhadra and James A. Birchler},
  journal={Cell},
  year={1997},
  volume={90},
  pages={479-490}
}
View on Elsevier
cell.com
247 Citations
Highly Influential Citations
6
Background Citations
80
Methods Citations
9

Figures from this paper

247 Citations

Citation Type

Citation Type

RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila.
Cosuppression of Nonhomologous Transgenes in Drosophila Involves Mutually Related Endogenous Sequences
New insights on homology-dependent silencing of I factor activity by transgenes containing ORF1 in Drosophila melanogaster.
TLDR
The relevance of the described regulation to the repression of I factors in I is discussed, suggesting the involvement of a homology-dependent trans-silencing mechanism.
A correlation between Long noncoding RNA and unpaired DNA silencing in Drosophila
TLDR
It is suggested that functional RNA-Piwi complex might create a silencing driven chromatin configuration by accumulating histone modifying enzymes at the Adh-w promoter target, which represents a novel mechanism to repress new transposon and foreign DNA insertions for protection of genome integrity.
Homology-dependent gene silencing in Paramecium.
TLDR
For the two multigene families that encode proteins that coassemble to build up complex subcellular structures the analysis presented herein provides the first experimental evidence that the members of these gene families are not functionally redundant.
Cosuppression of I transposon activity in Drosophila by I-containing sense and antisense transgenes.
TLDR
The data presented strongly support models in which the repressing effect of antisense transcripts involves the same mechanisms as cosuppression by sense constructs and emphasize the role of symmetrically acting nucleic acid structures in mediating repression.
Arabidopsis Mutants Impaired in Cosuppression
TLDR
The results indicate that sgs mutations act in trans to impede specifically transgene-induced post-transcriptional gene silencing.
Transgene-mediated co-suppression of DNA topoisomerase-1 gene in Caenorhabditis elegans.
TLDR
The findings suggest that transgene-mediated co-suppression facilitates the silencing of the specific genes and the study of gene function in vivo.
Transgene silencing in monocots
Plant gene silencing was originally thought to be a quirk of transformation procedures, but is now recognized to be a facet of vitally important gene regulatory systems, present in all organisms.…
Artificial and epigenetic regulation of the I factor, a nonviral retrotransposon of Drosophila melanogaster.
TLDR
The existence of the maternal effect and the genuine existence of a paternal effect argue for the occurrence, in the IF regulation pathway, of a cellular memory transmitted through mitosis, as well as through male and female meiosis, and akin to epigenetic phenomena.
...
...

References

SHOWING 1-10 OF 57 REFERENCES
Transgene‐mediated suppression of chalcone synthase expression in Petunia hybrida results from an increase in RNA turnover
TLDR
The data suggest that properties of the chs transgene locus other than the expression level are important for inducing co-suppression, and the possible role of antisense RNA, which was detected in all transformants, ectopic pairing and the structure of the integrated T-DNAs in the mechanism of the selective increase in chs RNA turnover are discussed.
Allelic pairing and gene regulation: A model for the zeste-white interaction in Drosophila melanogaster.
  • J. Jack, B. Judd
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1979
Data are presented that indicate that the z locus of Drosophila melanogaster represses w locus activity, but the repression is effective only on paired or physically adjacent w loci. Various mutant…
Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila
Transformation of white locus DNA in Drosophila: Dosage compensation, zeste interaction, and position effects
Deletions at intervening sequence splice sites in the alcohol dehydrogenase gene of Drosophila.
TLDR
The cloned mutant Adh genes, as well as the wild-type gene, are capable of promoting correct specific transcription initiation in HeLa cell nuclear extracts in vitro and it is concluded that Adhfn4 and AdHfn6 are defective in RNA processing.
A trans-acting regulatory gene that inversely affects the expression of the white, brown and scarlet loci in Drosophila.
TLDR
It is demonstrated that a single regulatory gene can exert an inverse effect on a target structural locus, a situation postulated from segmental aneuploid studies of gene expression and dosage compensation.
The effect of chromosomal position on the expression of the drosophila xanthine dehydrogenase gene
A Polycomb response element in the Ubx gene that determines an epigenetically inherited state of repression.
TLDR
A regulatory element in the Ubx gene that responds to Pc‐G and trx‐G genes is identified and establishes a repressive complex that keeps enhancers repressed in cells in which they were originally repressed and maintains this state through many cell divisions.
Cosuppression, Flower Color Patterns, and Metastable Gene Expression States
TLDR
Pigment transgenes elicit metastable cosuppression patterns organized by flower morphology, which reflect regulatory states that respond to morphological features and are labile to physiology and development.
Polycomb and polyhomeotic are constituents of a multimeric protein complex in chromatin of Drosophila melanogaster.
TLDR
Using immunoprecipitation and double‐immunofluorescence labelling, it is shown that two members of the Pc‐G, Polycomb and polyhomeotic, are constituents of a soluble multimeric protein complex.
...
...