Cost-effective frozen master mix modification of a commercial methicillin-resistant Staphylococcus aureus PCR assay.

Abstract

The expense inherent to molecular diagnostics may be an overriding concern for a variety of clinical laboratories in the development of PCR-based methicillin-resistant Staphylococcus aureus (MRSA) active surveillance programs. BD GeneOhm MRSA assay master mix was reconstituted, aliquoted into SmartCycler tubes in 25-microl volumes, and frozen at -70 degrees… (More)
DOI: 10.1128/JCM.00506-09

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