Cosolvent and crowding effects on the polymerization kinetics of actin.

  title={Cosolvent and crowding effects on the polymerization kinetics of actin.},
  author={Christopher Rosin and Paul Hendrik Schummel and Roland H. A. Winter},
  journal={Physical chemistry chemical physics : PCCP},
  volume={17 13},
We studied the effects of kosmotropic and chaotropic cosolvents, trimethylamine-N-oxide (TMAO) and urea, as well as crowding agents (dextran) on the polymerization reaction of actin. Time-lapse fluorescence intensity and anisotropy experiments were carried out to yield information about the kinetics of the polymerization process. To also quantitatively describe the effects, cosolvents and crowding impose on the underlying rate constants of the G-to-F-transformation, an integrative stochastic… 

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