Cortisol binding in cultured bovine mammary cells.

@article{Tucker1971CortisolBI,
  title={Cortisol binding in cultured bovine mammary cells.},
  author={Hugh A. Tucker and B. L. Larson and Jack Gorski},
  journal={Endocrinology},
  year={1971},
  volume={89 1},
  pages={
          152-60
        }
}
Bovine mammary cells cultured in vitro were incubated with varying levels of 3H-cortisol and various unlabeled steroids. These mammary cells possessed 2 components which had a high affinity (Kd≍5×lO8 to 2×109M) for the uptake of cortisol. There were approximately 7500 specific binding sites per mammary cell. The incorporation of 3H-cortisol into the mammary cell is inhibited by many (but not all) corticoids, by progesterone, by 17α-OH-progesterone and by preheating to 100 C for 10 min. It is… CONTINUE READING

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