Corticotropin Releasing Factor (CRF) Binding Protein: A Novel Regulator of CRF and Related Peptides

  title={Corticotropin Releasing Factor (CRF) Binding Protein: A Novel Regulator of CRF and Related Peptides},
  author={Dominic P. Behan and Errol B. de Souza and Philip J. Lowry and Ellen Potter and Paul F Sawchenko and Wylie W. Vale},
  journal={Frontiers in Neuroendocrinology},
A 37-kDa corticotropin releasing factor (CRF) binding protein (CRF-BP) was purified from human plasma by repeated affinity purification and subsequently sequenced and cloned. The human and rat CRF-BP cDNAs encode proteins of 322 amino acids with one putative signal sequence, one N-glycosylation site, and 10 conserved cysteines. Human CRF-BP binds human CRF with high affinity but has low affinity for the ovine peptide. In contrast, sheep CRF-BP binds human and ovine CRF with high affinity. The… 

Measurement of corticotropin-releasing factor (CRF), CRF-binding protein (CRF-BP), and CRF/CRF-BP complex in human plasma by two-site enzyme-linked immunoabsorbant assay.

The ELISAs in combination with high affinity h CRF-BP ligands for measuring bound and free hCRF in human plasma are validated and the utility of these assays for further determining alterations in peripheral CRF in conditions such as pregnancy is suggested.

Corticotropin-Releasing Factor (CRF) and Endocrine Responses to Stress: CRF Receptors, Binding Protein, and Related Peptides

  • A. TurnbullC. Rivier
  • Biology
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1997
Recent findings with respect to CRF, its receptors, binding protein, and CRF-related peptides are described, which provide further insights into the role and mechanisms of CRF action in stress responses.

Heterogeneity of the human corticotropin-releasing factor-binding protein.

Evidence is presented for a C-terminally truncated form of the native binding protein in the plasma of subjects suffering from rheumatoid arthritis, which may parallel the in vitro truncation.

Widespread tissue distribution and diverse functions of corticotropin-releasing factor and related peptides.

Corticotropin-releasing factor inhibits luteinizing hormone-stimulated P450c17 gene expression and androgen production by isolated thecal cells of human ovarian follicles.

The ovarian CRF system may function as autocrine regulators for androgen biosynthesis in the thecal cell compartment to maintain optimal substrate for estrogen biosynthesis by granulosa cells.

Sequences, expression patterns and regulation of the corticotropin-releasing factor system in a teleost.

Expression of genes encoding corticotropin-releasing factor (CRF), type 1 CRF receptor, and CRF-binding protein and localization of the gene products in the human ovary.

The parallel increases in intensity of CRF, CRF-R1, and 17 alpha-hydroxylase proteins and gene expression with follicular maturation suggest that the intraovarian CRF system may play an autocrine role in androgen biosynthesis with a downstream effect on estrogen production by the granulosa cells.

Characterization of the human corticotropin-releasing factor2(a) receptor promoter: regulation by glucocorticoids and the cyclic adenosine 5'-monophosphate pathway.

The present studies are the first to characterize the transcriptional regulation of the human CRF2(a), the predominantCRF2 isoform in brain, and identify the functional cAMP response element and glucocorticoid response element cis-regulatory elements within these regions were identified using a combination of site-directed mutagenesis and EMSAs.