Corticotrophin-releasing hormone (CRH)-binding protein interference with CRH antibody binding: implications for direct CRH immunoassay.

@article{Linton1995CorticotrophinreleasingH,
  title={Corticotrophin-releasing hormone (CRH)-binding protein interference with CRH antibody binding: implications for direct CRH immunoassay.},
  author={Elizabeth A. Linton and Anthony V Perkins and Phillip L. Hagan and Stephen Poole and Adrian F. Bristow and Fred Tilders and Roger Corder and Charles D A Wolfe},
  journal={The Journal of endocrinology},
  year={1995},
  volume={146 1},
  pages={45-53}
}
Direct immunoassay of plasma corticotrophin-releasing hormone (CRH) is potentially subject to interference from high levels of CRH-binding protein (CRH-BP) that exist in the human circulation. In this study, we tested the effect of CRH-free, native CRH-BP (6.4 nmol/l) purified from human plasma, CRH-BP diluent alone, normal human plasma (containing 5.8 nmol endogenous CRH-BP/l) and normal sheep plasma (containing no CRH-BP) on the binding of 125I-labelled CRH tracer to five N-terminal and four… CONTINUE READING

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