Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1

  title={Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1},
  author={Safia Omer and Samuel R Greenberg and Wei-Lih Lee},
Cortical dynein generates pulling forces via microtubule (MT) end capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with endoplasmic reticulum (ER) and mitochondria to facilitate spindle positioning across the mother-bud neck, but direct evidence for how these cortical contacts regulate dynein-dependent pulling forces is lacking. We show that loss of Scs2/Scs22, ER tethering proteins, resulted in defective Num1… 

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