Correlative two-photon and serial block face scanning electron microscopy in neuronal tissue using 3D near-infrared branding maps.

@article{Lees2017CorrelativeTA,
  title={Correlative two-photon and serial block face scanning electron microscopy in neuronal tissue using 3D near-infrared branding maps.},
  author={Robert M. Lees and Christopher J. Peddie and Lucy M. Collinson and Michael C. Ashby and Paul Verkade},
  journal={Methods in cell biology},
  year={2017},
  volume={140},
  pages={245-276}
}
Linking cellular structure and function has always been a key goal of microscopy, but obtaining high resolution spatial and temporal information from the same specimen is a fundamental challenge. Two-photon (2P) microscopy allows imaging deep inside intact tissue, bringing great insight into the structural and functional dynamics of cells in their physiological environment. At the nanoscale, the complex ultrastructure of a cell's environment in tissue can be reconstructed in three dimensions… CONTINUE READING
Related Discussions
This paper has been referenced on Twitter 4 times. VIEW TWEETS

Citations

Publications citing this paper.

Precise and economic FIB/SEM for CLEM: with 2 nm voxels through mitosis

Histochemistry and Cell Biology • 2018
View 13 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…