Correlation of rRNA gene amplicon pyrosequencing and bacterial culture for microbial compositional analysis of faecal samples from elderly Irish subjects.

@article{OSullivan2011CorrelationOR,
  title={Correlation of rRNA gene amplicon pyrosequencing and bacterial culture for microbial compositional analysis of faecal samples from elderly Irish subjects.},
  author={Orla O'Sullivan and Mair{\'e}ad Coakley and Bhuvaneswari Lakshminarayanan and Marcus J. Claesson and Catherine Stanton and Paul W O'Toole and R Paul Ross},
  journal={Journal of applied microbiology},
  year={2011},
  volume={111 2},
  pages={467-73}
}
AIMS The aim of this investigation was to establish the degree of correlation between measurements from culture-dependent microbiological techniques and from next generation sequencing technologies. METHODS AND RESULTS Data generated by both techniques were collected from faecal samples from 185 elderly Irish people involved in the ongoing ELDERMET study (http://eldermet.ucc.ie). The results for three groups of intestinal bacteria were compared. Bifidobacterium sp., Lactobacillus sp. and… CONTINUE READING

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