Correlation of increased intraacrosomal pH with the hamster sperm acrosome reaction.

@article{Working1983CorrelationOI,
  title={Correlation of increased intraacrosomal pH with the hamster sperm acrosome reaction.},
  author={Peter K. Working and Stanley Meizel},
  journal={The Journal of experimental zoology},
  year={1983},
  volume={227 1},
  pages={97-107}
}
This study describes investigations of the importance of intraacrosomal pH in the hamster sperm acrosome reaction (AR). Washed cauda epididymal sperm were capacitated in vitro in a medium containing 2 mM Ca2+, 144 mM Na+, and 3 mM K+. Such sperm underwent a significant increase in the number of AR within 10 min after the addition of the Mg2+-ATPase (adenosine triphosphatase) inhibitors DCCD (20 microM) or NBD-Cl (10 microM) or the proton ionophore FCCP (6 micrograms/ml) at 3.5 hr of incubation… CONTINUE READING

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