Correlation of high-throughput pregnane X receptor (PXR) transactivation and binding assays.

@article{Zhu2004CorrelationOH,
  title={Correlation of high-throughput pregnane X receptor (PXR) transactivation and binding assays.},
  author={Zhengrong Zhu and Sean W Kim and Taosheng Chen and Jane Lin and Aneka Bell and James A Bryson and Yves P Dubaquie and N. Yan and Joseph Yanchunas and Dianlin Xie and Robert H. Stoffel and Michael W. Sinz and Kenneth E Dickinson},
  journal={Journal of biomolecular screening},
  year={2004},
  volume={9 6},
  pages={533-40}
}
Pregnane X receptor (PXR) transactivation and binding assays have been developed into high-throughput assays, which are robust and reproducible (Z' > 0.5). For most compounds, there was a good correlation between the results of the transactivation and binding assays. EC(50) values of compounds in the transactivation assay correlated reasonably well with their IC(50) values in the binding assay. However, there were discrepancies with some compounds showing high binding affinity in the binding… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 20 extracted citations

Similar Papers

Loading similar papers…