Correlation of cytotoxicity with incorporation of ara-C into DNA.

  title={Correlation of cytotoxicity with incorporation of ara-C into DNA.},
  author={Donald Kufe and Pierre P. Major and E. Michael Egan and G. Peter Beardsley},
  journal={The Journal of biological chemistry},
  volume={255 19},
We have employed cesium sulfate density gradient analysis to monitor the incorporation of 1-beta-D-arabinofuranosylcytosine (ara-C) into L1210 cellular nucleic acid. This methodology permits a distinct separation of RNA and DNA under nondegrading conditions. The results demonstrate specific incorporation of ara-C into DNA with less than 1% of total incorporation into nucleic acid being detectable in RNA. Moreover, we have found a highly significant relationship (p < 0.0001) between the… CONTINUE READING