Correlation between processing efficiency for ribonuclease P minimal substrates and conformation of the nucleotide -1 at the cleavage position.

@article{Zuleeg2001CorrelationBP,
  title={Correlation between processing efficiency for ribonuclease P minimal substrates and conformation of the nucleotide -1 at the cleavage position.},
  author={T Zuleeg and Anna Hansen and Thomas Pfeiffer and Helmut Sch{\"u}bel and Roland Kreutzer and Roland K. Hartmann and Steven Limmer},
  journal={Biochemistry},
  year={2001},
  volume={40 11},
  pages={3363-9}
}
It is demonstrated that acceptor stem duplexes derived from native tRNAs which contain a three-nucleotide extension at the 5'-terminus of mature tRNA are minimal substrates for ribonuclease P from both Escherichia coli and Bacillus subtilis. Variants with a cytidine at position -1 are most efficiently processed whereas the G -1 variant represents a comparatively poor substrate. An A -1 acceptor stem variant is a slightly better substrate than the G -1 variant though generally distinctly less… CONTINUE READING