Correlation between in vitro stability and in vivo performance of anti-GCN4 intrabodies as cytoplasmic inhibitors.

@article{Wrn2000CorrelationBI,
  title={Correlation between in vitro stability and in vivo performance of anti-GCN4 intrabodies as cytoplasmic inhibitors.},
  author={A W{\"o}rn and Adrian Auf der Maur and Dominik Escher and Annemarie Honegger and Alcide Barberis and Andreas Pl{\"u}ckthun},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 4},
  pages={2795-803}
}
A cellular assay system for measuring the activity of cytoplasmically expressed anti-GCN4 scFv fragments directed against the Gcn4p dimerization domain was established in the budding yeast Saccharomyces cerevisiae. The inhibitory potential of different constitutively expressed anti-GCN4 scFv intrabodies was monitored by measuring the activity of beta-galactosidase expressed from a GCN4-dependent reporter gene. The in vivo performance of these scFv intrabodies in specifically decreasing reporter… CONTINUE READING

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Stability Engineering of Antibody Single-chain Fv Fragments

Arne WoÈ, Andreas PluÈ ckthun
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Protein science : a publication of the Protein Society • 2002
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Sequences of Proteins of Immunological Interest, NIH Publication 91-3242

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