Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity.

@article{Schorb2014CorrelatedCA,
  title={Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity.},
  author={Martin Schorb and John A. G. Briggs},
  journal={Ultramicroscopy},
  year={2014},
  volume={143},
  pages={24-32}
}
Performing fluorescence microscopy and electron microscopy on the same sample allows fluorescent signals to be used to identify and locate features of interest for subsequent imaging by electron microscopy. To carry out such correlative microscopy on vitrified samples appropriate for structural cryo-electron microscopy it is necessary to perform fluorescence microscopy at liquid-nitrogen temperatures. Here we describe an adaptation of a cryo-light microscopy stage to permit use of high… CONTINUE READING

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