Correction of the apical membrane chloride permeability defect in polarized cystic fibrosis airway epithelia following retroviral-mediated gene transfer.

@article{Olsen1992CorrectionOT,
  title={Correction of the apical membrane chloride permeability defect in polarized cystic fibrosis airway epithelia following retroviral-mediated gene transfer.},
  author={John C. Olsen and Larry G. Johnson and M Jackson Stutts and Bal{\'a}zs Sarkadi and James R. Yankaskas and R. Swanstrom and Richard C. Boucher},
  journal={Human gene therapy},
  year={1992},
  volume={3 3},
  pages={253-66}
}
We are studying the introduction and expression of the normal cystic fibrosis transmembrane conductance regulator (CFTR) cDNA into cultured human airway epithelial cells as a model for gene therapy of cystic fibrosis. In this paper, we show that the chloride transport defect at the apical membrane is corrected in vitro in differentiated ion-transporting CF airway epithelial cells that exhibit polarized properties similar to those found in vivo. Using a retroviral vector containing a copy of the… CONTINUE READING

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