Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide–based retroviral vector

@article{Szymczak2004CorrectionOM,
  title={Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide–based retroviral vector},
  author={Andrea L. Szymczak and Creg J Workman and Yao Wang and Kate M Vignali and Smaroula Dilioglou and Elio Fausto Vanin and Dario A A Vignali},
  journal={Nature Biotechnology},
  year={2004},
  volume={22},
  pages={589-594}
}
Attempts to generate reliable and versatile vectors for gene therapy and biomedical research that express multiple genes have met with limited success. Here we used Picornavirus 'self-cleaving' 2A peptides, or 2A-like sequences from other viruses1,2,3, to generate multicistronic retroviral vectors with efficient translation of four cistrons. Using the T-cell receptor:CD3 complex as a test system, we show that a single 2A peptide–linked retroviral vector can be used to generate all four CD3… CONTINUE READING
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