Correcting the Effects of −20 °C Storage and Aliquot Size on Erythrocyte Fatty Acid Content in the Women’s Health Initiative

@article{Pottala2012CorrectingTE,
  title={Correcting the Effects of −20 °C Storage and Aliquot Size on Erythrocyte Fatty Acid Content in the Women’s Health Initiative},
  author={James V. Pottala and Mark A. Espeland and Jason Polreis and Jennifer G. Robinson and William S. Harris},
  journal={Lipids},
  year={2012},
  volume={47},
  pages={835-846}
}
Red blood cell (RBC) fatty acid (FA) patterns have been shown to predict risk for cardiovascular and other chronic diseases. As part of a project analyzing RBC samples from the Women’s Health Initiative Memory Study (WHIMS) we observed implausibly low levels of highly unsaturated fatty acids (HUFA) suggestive of degradation. This was hypothesized to be due to short term storage (<1 month) at −20 °C during sample aliquoting. The purpose of this study was to measure the extent of degradation that… CONTINUE READING