Correct transcription of a cloned mouse immunoglobulin gene in vivo.


We have obtained correct transcripts from the mouse immunoglobulin lambda I light chain gene on transfection into human HeLa cells. Linkage to simian virus 40 (SV40) DNA containing a transcriptional "enhancer" element was required to raise lambda-chain gene transcription to a detectable level in our transient-expression assay. The transcripts had the same 5' end as authentic lambda I mRNA when the SV40 enhancer element was 150 base pairs upstream from the cap site. The situation was different when the lambda-chain gene promoter was separated from the SV40 sequences by more than 1 kilobase pair of spacer DNA; then, lambda-chain gene transcripts were not correctly initiated in human HeLa, monkey CV-1, and mouse 3T6 cells. In this respect, the lambda-chain gene behaves differently from the rabbit beta-globin gene, which can be activated by the SV40 enhancer over distances of several kilobases.


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@article{Picard1983CorrectTO, title={Correct transcription of a cloned mouse immunoglobulin gene in vivo.}, author={Didier Picard and Walter Schaffner}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1983}, volume={80 2}, pages={417-21} }