Correct interpretation of comprehensive phosphorylation dynamics requires normalization by protein expression changes.

@article{Wu2011CorrectIO,
  title={Correct interpretation of comprehensive phosphorylation dynamics requires normalization by protein expression changes.},
  author={Ronghu Wu and Noah Dephoure and Wilhelm Haas and Edward L. Huttlin and Bo Zhai and Mathew E. Sowa and Steven P Gygi},
  journal={Molecular & cellular proteomics : MCP},
  year={2011},
  volume={10 8},
  pages={M111.009654}
}
The interpretation of quantitative phosphoproteomics studies is complicated because each differential phosphorylation event integrates both changes in protein expression and phosphorylation. Here we investigated this phenomenon by performing parallel comparisons of protein expression and phosphorylation in S. cerevisiae. In each of two experiments comparing yeast mutants bearing deletions in FUS3 or STE7 with their wild-type counterparts, we quantified over 4100 proteins, including all members… CONTINUE READING

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