Correct integration of retroviral DNA in vitro

  title={Correct integration of retroviral DNA in vitro},
  author={Patrick O. Brown and Bruce Bowerman and Harold Varmus and J. M. Bishop},

Relationship of avian retrovirus DNA synthesis to integration in vitro

Analysis of integration intermediates revealed that the strand transfer mechanisms of both reactions were identical to those of retroviruses and some transposons, and most cytoplasmic viral DNA appears to be incomplete and further DNA synthesis is required for integration in vitro.

Human immunodeficiency virus integration in a cell-free system

An in vitro method for studying the biochemistry of human immunodeficiency virus integration by using extracts from HIV-infected cells showed that HIV integration in vitro accurately reproduces the in vivo process.

Retroviral DNA Integration

The molecular mechanism of retroviral DNA integration is reviewed, with an emphasis on reaction chemistries and architectures of the nucleoprotein complexes involved, and the latest advances on anti-integrase drug development for the treatment of AIDS are discussed.

Retroviral DNA Integration

At least two host proteins, HMG-I(Y) and BAF, have been shown to increase the efficiency of the integration reaction, and small duplications of the host DNA, characteristic of the viral IN, are found at the sites of insertion.

Integration of retroviral DNA.

  • P. Brown
  • Biology
    Current topics in microbiology and immunology
  • 1990
Integration of a DNA copy of the viral genome into host cellular DNA is an essential step in the life cycle of retroviruses and defines the critical switch in the viral life cycle from mere subsistence to multiplication.

Molecular mechanism of retroviral DNA integration.

Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro

The DNA sequences at the ends of the linear proviral precursor that are required for integration in the presence of MoMLV integration protein in vitro are characterized and it is inferred that a 3'-terminal A residue was preferred for integration.

A nucleoprotein complex mediates the integration of retroviral DNA.

Analysis of the native state of viral DNA in cells acutely infected by murine leukemia virus shows that the viral capsid protein is part of the active nucleoprotein complex, but recognition of the complex by only a subset of anti-capsid sera implies that the protein is constrained conformationally.



The retrovirus pol gene encodes a product required for DNA integration: identification of a retrovirus int locus.

  • A. PanganibanH. Temin
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
The 3' end of the pol gene serves as an "int" locus and encodes a protein mediating integration of retrovirus DNA through interaction with att, showing that the att- virus provided a transacting function allowing integration of viral DNA derived from the mutant bearing a wild-type att site.

Integration and expression of several molecular forms of Rous sarcoma virus DNA used for transfection of mouse cells

Analysis of viral polyadenylated RNA and patterns of viral DNA in transformed cells indicated that viral DNA can be integrated and expressed without regard to LTR sequences, with adjacent host DNA presumably supplying signals required for the promotion and processing of functional src mRNA.

A mutant murine leukemia virus with a single missense codon in pol is defective in a function affecting integration.

  • L. DonehowerH. Varmus
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
It is concluded that the MuLV-SF1 pol gene is defective for a function that is required for normal integrative recombination and dissociable from DNA synthesis.

Site-specific recombination of yeast 2-micron DNA in vitro.

This work has cloned the FLP gene of 2-micron DNA under control of a strong yeast promoter and transformed yeast cells with a plasmid containing the cloned FLPGene, a site-specific recombinase encoded by bacteriophage P1.

Sequence of retrovirus provirus resembles that of bacterial transposable elements

The nucleotide sequences of the terminal regions of an infectious integrated retrovirus cloned in the modified λ phage cloning vector Charon 4A have been elucidated and resemble that of a transposable element and are consistent with the protovirus hypothesis that retroviruses evolved from the cell genome.