Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line.

@article{Goldberg1983CorrectDE,
  title={Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line.},
  author={Daniel A Goldberg and James W Posakony and Tom Maniatis},
  journal={Cell},
  year={1983},
  volume={34 1},
  pages={59-73}
}
We have used P-element-mediated transformation to introduce a cloned Drosophila alcohol dehydrogenase (Adh) gene into the germ line of ADH null flies. Six independent transformants expressing ADH were identified by their acquired resistance to ethanol. Each transformant carries a single copy of the cloned Adh gene in a different chromosomal location. Four of the six transformant lines exhibit normal Adh expression by the following criteria: quantitative levels of ADH enzyme activity in larvae… CONTINUE READING