Coronavirus Multiplication Strategy I. Identification and Characterization of Virus-Specified RNA

@article{Stern1980CoronavirusMS,
  title={Coronavirus Multiplication Strategy I. Identification and Characterization of Virus-Specified RNA},
  author={D. Stern and S. Kennedy},
  journal={Journal of Virology},
  year={1980},
  volume={34},
  pages={665 - 674}
}
We examined the synthesis of intracellular RNA in primary chicken embryo kidney cells infected with the avian coronavirus infectious bronchitis virus. Infected cells were labeled with 32Pi in the presence of actinomycin D for the duration of the viral multiplication cycle, and nucleic acids were extracted, denatured, and analyzed on agarose slab gels. Six major RNA species were found. None of these RNAs was found in extracts of mock-infected cells. All six of the virus-specified RNAs… Expand
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