Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae.

Abstract

The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, high copy number is associated with low instability; (iii) it is difficult to reconcile this variability with a strict and direct system of copy number control; (iv) instabilities are much higher than expected from random partition and the observed copy numbers: this may imply partition which is less efficient than random. Even so, (v) the partitioning of 2-micron circle-like plasmids is more efficient than that of ARS-based plasmids, which hints at the existence of a system for the (inefficient) distribution of 2-micron circles.

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@article{Futcher1984CopyNA, title={Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae.}, author={A. Bruce Futcher and Brian Cox}, journal={Journal of bacteriology}, year={1984}, volume={157 1}, pages={283-90} }