Copper transport and kinetics in cultured C6 rat glioma cells.

  title={Copper transport and kinetics in cultured C6 rat glioma cells.},
  author={Yongchang Qian and Evelyn Tiffany-Castiglioni and E. D. Harris},
  journal={The American journal of physiology},
  volume={269 4 Pt 1},
C6 rat glioma cells accumulate and efflux 67Cu. Both processes showed saturation kinetics with increasing 67Cu concentration. The Michaelis constant (Km) for uptake was 0.63 +/- 0.14 microM; maximum velocity (Vmax) was 3.29 +/- 0.57 pmol protein-1.min-1. The Km for efflux was 0.15 +/- 0.06 microM; Vmax was 1.08 +/- 0.71 pmol protein-1.min-1. p-Chloromercuribenzoate (p-CMB) totally blocked 67Cu efflux but had no effect on Km or Vmax of uptake. Total 67Cu in the cell after 50 min was… CONTINUE READING

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