Copper staining: a five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels.

@article{Lee1987CopperSA,
  title={Copper staining: a five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels.},
  author={Chan Ping Lee and Alan E. Levin and Daniel Branton},
  journal={Analytical biochemistry},
  year={1987},
  volume={166 2},
  pages={308-12}
}
We present a new method for visualizing proteins electrophoresed in sodium dodecyl sulfate-polyacrylamide gels. After electrophoresis, gels are incubated in CuCl2 to produce a negative image of colorless protein bands against a semiopaque background. Gels are stained completely within 5 min, do not require destaining, and can be stored indefinitely without loss of the image. Because proteins are not permanently fixed within the gel, they can be quantitatively eluted after chelation of Cu with… CONTINUE READING
Highly Influential
This paper has highly influenced 11 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 78 extracted citations

Similar Papers

Loading similar papers…