Coping with replication 'train wrecks' in Escherichia coli using Pol V, Pol II and RecA proteins.

@article{Goodman2000CopingWR,
  title={Coping with replication 'train wrecks' in Escherichia coli using Pol V, Pol II and RecA proteins.},
  author={Myron F. Goodman},
  journal={Trends in biochemical sciences},
  year={2000},
  volume={25 4},
  pages={189-95}
}
DNA replication machineries tend to stall when confronted with damaged DNA template sites, causing the biochemical equivalent of a major 'train wreck'. A newly discovered bacterial DNA polymerase, Escherichia coli Pol V, acting in conjunction with the RecA protein, can exchange places with the stalled replicative Pol III core and catalyse 'error-prone' translesion synthesis. In contrast to Pol V-catalysed 'brute-force, sloppier copying', another SOS-induced DNA polymerase, Pol II, plays a… CONTINUE READING