Coordination of multiple enzyme activities by a single PCNA in archaeal Okazaki fragment maturation

@inproceedings{Beattie2012CoordinationOM,
  title={Coordination of multiple enzyme activities by a single PCNA in archaeal Okazaki fragment maturation},
  author={Thomas R. Beattie and Stephen D Bell},
  booktitle={The EMBO journal},
  year={2012}
}
Chromosomal DNA replication requires one daughter strand-the lagging strand-to be synthesised as a series of discontinuous, RNA-primed Okazaki fragments, which must subsequently be matured into a single covalent DNA strand. Here, we describe the reconstitution of Okazaki fragment maturation in vitro using proteins derived from the archaeon Sulfolobus solfataricus. Six proteins are necessary and sufficient for coupled DNA synthesis, RNA primer removal and DNA ligation. PolB1, Fen1 and Lig1… CONTINUE READING
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