Coordinate Binding of ATP and Origin DNA Regulates the ATPase Activity of the Origin Recognition Complex

@article{Klemm1997CoordinateBO,
  title={Coordinate Binding of ATP and Origin DNA Regulates the ATPase Activity of the Origin Recognition Complex},
  author={Richard D Klemm and Richard J. Austin and Stephen P Bell},
  journal={Cell},
  year={1997},
  volume={88},
  pages={493-502}
}
The Origin Recognition Complex (ORC) is a six-protein assembly that specifies the sites of DNA replication initiation in S. cerevisiae. Origin recognition by ORC requires ATP. Here, we demonstrate that two subunits, Orc1p and Orc5p, bind ATP and that Orc1p also hydrolyzes ATP. ATP binding and hydrolysis by Orc1p are both regulated by origin DNA in a sequence-specific manner. ATP binding to Orc1p, but not ATP hydrolysis, is responsible for the ATP dependence of the ORC-origin interaction… CONTINUE READING