Cooperative calcium binding and calmodulin regulation in the calcium-dependent adenosine triphosphatase purified from the erythrocyte membrane.

Abstract

Calcium binding to calcium-dependent ATPase purified from erythrocyte membrane was assessed by measurements of the ATPase intrinsic fluorescence. Calcium-binding isotherms obtained by fluorescence titration are identical to curves representing the Ca2+-concentration dependence of ATPase activity, and demonstrate that cooperativity is in fact a feature of… (More)

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