Cooperative calcium binding and ATPase activation in sarcoplasmic reticulum vesicles.

  title={Cooperative calcium binding and ATPase activation in sarcoplasmic reticulum vesicles.},
  author={Giuseppe Inesi and M Kurzmack and Carol Coan and Dale E. A. Lewis},
  journal={The Journal of biological chemistry},
  volume={255 7},
High affinity calcium binding which is involved in ATPase activation was measured following equilibration of sarcoplasmic reticulum vesicles with Ca2+ buffers in chromatography columns. Binding occurred with ir,,, = 2.3 X lo6 M” and with a maximal level of 8 to 10 m o l per mg of protein. This value corresponds to 2 calcium ions per ATPase phosphorylation site, since the maximal level of phosphorylated enzyme intermediate obtained in the presence of ATP was 4 to 5 nmol per mg of protein… CONTINUE READING


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