Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy.

@article{Gugel2004Cooperative4E,
  title={Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy.},
  author={Hilmar Gugel and Joerg Bewersdorf and Stefan Jakobs and Johann Engelhardt and R. H. Storz and Stefan W Hell},
  journal={Biophysical journal},
  year={2004},
  volume={87 6},
  pages={4146-52}
}
Although the addition of just the excitation light field at the focus, or of just the fluorescence field at the detector is sufficient for a three- to fivefold resolution increase in 4Pi-fluorescence microscopy, substantial improvements of its optical properties are achieved by exploiting both effects simultaneously. They encompass not only an additional expansion of the optical bandwidth, but also an amplified transfer of the newly gained spatial frequencies to the image. Here we report on the… CONTINUE READING
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Confocal and Two-Photon Microscopy

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