Convex lens-induced confinement for imaging single molecules.

  title={Convex lens-induced confinement for imaging single molecules.},
  author={Sabrina R. Leslie and Alexander P. Fields and Adam E Cohen},
  journal={Analytical chemistry},
  volume={82 14},
Fluorescence imaging is used to study the dynamics of a wide variety of single molecules in solution or attached to a surface. Two key challenges in this pursuit are (1) to image immobilized single molecules in the presence of a high level of fluorescent background and (2) to image freely diffusing single molecules for long times. Strategies that perform well by one measure often perform poorly by the other. Here, we present a simple modification to a wide-field fluorescence microscope that… CONTINUE READING

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