Conversion to docosahexaenoic acid-containing phosphatidylserine from squid skin lecithin by phospholipase D-mediated transphosphatidylation.

@article{Hosokawa2000ConversionTD,
  title={Conversion to docosahexaenoic acid-containing phosphatidylserine from squid skin lecithin by phospholipase D-mediated transphosphatidylation.},
  author={Masashi Hosokawa and Takuro Shimatani and Tomohiro Kanada and Yoshimasa Inoue and Koji Takahashi},
  journal={Journal of agricultural and food chemistry},
  year={2000},
  volume={48 10},
  pages={4550-4}
}
Phospholipase D (PLD)-mediated transphosphatidylation of squid skin lecithin with L-serine was examined to prepare docosahexaenoic acid-containing phosphatidylserine (DHA-PS). When a biphasic system with organic solvent and 0.2 M acetate buffer (pH 5.5) was used, PS synthesis was significantly affected by the amount of 3.4 M L-serine-containing acetate buffer. L-Serine concentration in the acetate buffer and choice of organic solvent were also crucial. In a typical reaction with 0.8 unit of PLD… CONTINUE READING

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