Conversion of oleic acid to 10-hydroxystearic acid by Nocardia paraffinae

@article{Latrasse1997ConversionOO,
  title={Conversion of oleic acid to 10-hydroxystearic acid by Nocardia paraffinae},
  author={Alain Latrasse and St{\'e}phanie Paitier and Bruno Lachot and Pascal Bonnarme and Gilles Feron and A. Durand and J. L. Le Qu{\'e}r{\'e}},
  journal={Biotechnology Letters},
  year={1997},
  volume={19},
  pages={715-718}
}
Resting cells of Nocardia paraffinaeCBS 255 58 anaerobically converted oleic acid to 10-hydroxystearic acid (optimum activity at 35°C and pH 7). Addition of oleic acid (0.22 mg/ml of medium), as an inducer, improved the hydroxylating activity. The biocatalyst was inactivated by sonication, lyophilization and heating, but kept 65% of its initial activity after five freeze-thawing treatments. This activity appeared to be located either in the cells or bound to the plasmic membrane. 

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