Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy

  title={Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy},
  author={Jem A. Efe and Simon A Hilcove and Janghwan Kim and Hongyan Zhou and Kunfu Ouyang and G. Wang and Ju Chen and Sheng Ding},
  journal={Nature Cell Biology},
Here we show that conventional reprogramming towards pluripotency through overexpression of Oct4, Sox2, Klf4 and c-Myc can be shortcut and directed towards cardiogenesis in a fast and efficient manner. With as little as 4 days of transgenic expression of these factors, mouse embryonic fibroblasts (MEFs) can be directly reprogrammed to spontaneously contracting patches of differentiated cardiomyocytes over a period of 11–12 days. Several lines of evidence suggest that a pluripotent intermediate… 

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