Conversion of Wine Strains of Saccharomyces cerevisiae to Heterothallism.

@article{Bakalinsky1990ConversionOW,
  title={Conversion of Wine Strains of Saccharomyces cerevisiae to Heterothallism.},
  author={Alan T. Bakalinsky and Richard Snow},
  journal={Applied and environmental microbiology},
  year={1990},
  volume={56 4},
  pages={849-57}
}
A general method to convert homothallic strains of the yeast Saccharomyces cerevisiae to heterothallism is described which is applicable to genetically well-behaved diploids, as well as to strains that sporulate poorly or produce few viable and mating-competent spores. The heterothallic (ho) allele was introduced into three widely used wine strains through spore x cell hybridization. The resultant hybrids were sporulated, and heterothallic segregants were isolated for use in successive… CONTINUE READING

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