Conversion of L‐tryptophan to serotonin and melatonin in human melanoma cells

@article{Slominski2002ConversionOL,
  title={Conversion of L‐tryptophan to serotonin and melatonin in human melanoma cells},
  author={Andrzej T. Slominski and Igor V Semak and Alexander V. Pisarchik and Trevor W. Sweatman and Andre Szczesniewski and Jacobo Wortsman},
  journal={FEBS Letters},
  year={2002},
  volume={511}
}
The expanding roles of 1‐methyl‐tryptophan (1‐MT): in addition to inhibiting kynurenine production, 1‐MT activates the synthesis of melatonin in skin cells
TLDR
It is reported that melatonin biosynthesis is driven by 1‐methyl‐tryptophan (1‐MT), a competitive inhibitor of IDO1, in human fibroblasts, melanocytes and melanoma cells, and it was observed that 1‐MT, as well melatonin, inhibited the proliferation of human melanomas cells.
Serotoninergic and melatoninergic systems are fully expressed in human skin
TLDR
Examination of cutaneous expression of genes and enzymes responsible for the multistep conversion of tryptophan to serotonin and further to melatonin provides evidence that the human skin expresses intrinsic serotonin and melatonin biosynthesis pathways.
Metabolism of melatonin and biological activity of intermediates of melatoninergic pathway in human skin cells
TLDR
The metabolism of melatonin and its endogenous production is cell type‐dependent and expressed in all three main cell populations of human skin and stimulate differentiation in human epidermis, indicating their key role in building the skin barrier.
Characterization of serotonin and N‐acetylserotonin systems in the human epidermis and skin cells
TLDR
A serotonin‐NAS system in human skin that is a part of local neuroendocrine system regulating skin homeostasis is characterized and produced endogenously in the epidermal, dermal, and adnexal compartments of human skin and in cultured skin cells.
Characterization of the serotoninergic system in the C57BL/6 mouse skin.
TLDR
Mouse skin has the molecular and biochemical apparatus necessary to produce and metabolize serotonin and N-acetylserotonin, and its activity is determined by topography, physiological status of the skin, cell type and mouse strain.
Tryptophan hydroxylase expression in human skin cells.
Up‐regulation of melanin synthesis by the antidepressant fluoxetine
TLDR
The results suggest that fluoxetine may hold a significant therapeutic potential for treating skin hypopigmentation disorders, and SR1A may serve as a novel target in modulating melanogenesis.
Functional activity of serotoninergic and melatoninergic systems expressed in the skin
TLDR
Skin cells represent a true target for the products of the serotoninergic/melatoninergic cutaneous pathway with their actions modulating cell proliferation or viability, according to cell type and culture conditions.
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TLDR
It is concluded that mammalian skin can acetylate serotonin to NAS and postulate that the NAS is further metabolized by the skin to form melatonin which is subsequently transformed to 5-MT.
Clinical chemistry of serotonin and metabolites.
Modification of melanogenesis in cultured human melanoma cells
TLDR
Since the hamster amelanotic melanoma line shows profound metabolic and cellular changes accompanying the induction of melanin synthesis by DMEM or L-tyrosine, it is tested whether the changes in pigmentation level in human melanoma cells are similarly accompanied by increased expression of tyrosinasc and MC1R genes.
A Sensitive Radiometric Assay for Tryptophan Hydroxylase Applicable to Crude Extracts
TLDR
A simple and convenient method for assay of tryptophan 5‐monooxygenase (hydroxylase), applicable to enzyme in all states of purification, gives results similar to those in a standard fluorimetric assay.
Identification and characterization of two isozymic forms of arylamine N-acetyltransferase in Syrian hamster skin.
TLDR
Two isozymes of arylamine N-acetyltransferase, designated NAT-1 and NAT-2, were detected on anion-exchange high-performance liquid chromatography analysis and raises new questions about the physiologic role of this enzyme in general and in the skin-specific functions in particular.
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TLDR
The role of Melatonin in Human Pathophysiology, in Oncology, and Melatonin Influences on Epilepsy are examined.
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