Conversion of AFLP markers to sequence-specific PCR markers in barley and wheat

@article{Shan1999ConversionOA,
  title={Conversion of AFLP markers to sequence-specific PCR markers in barley and wheat},
  author={Xiaohui Shan and Thomas K. Blake and Luther E. Talbert},
  journal={Theoretical and Applied Genetics},
  year={1999},
  volume={98},
  pages={1072-1078}
}
 Conversion of amplified fragment length polymorphisms (AFLPs) to sequence-specific PCR primers would be useful for many genetic-linkage applications. We examined 21 wheat nullitetrasomic stocks and five wheat-barley addition lines using 12 and 14 AFLP primer combinations, respectively. On average, 36.8% of the scored AFLP fragments in the wheat nullitetrasomic stocks and 22.3% in the wheat-barley addition lines could be mapped to specific chromosomes, providing approximately 461 chromosome… CONTINUE READING
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