Conventional and real-time polymerase chain reaction assessment of the fate of transgenic DNA in sheep fed Roundup Ready rapeseed meal.

@article{Alexander2006ConventionalAR,
  title={Conventional and real-time polymerase chain reaction assessment of the fate of transgenic DNA in sheep fed Roundup Ready rapeseed meal.},
  author={Trevor Alexander and Tim Reuter and Erasmus Kojo Okine and Ranjana Sharma and Tim A McAllister},
  journal={The British journal of nutrition},
  year={2006},
  volume={96 6},
  pages={997-1005}
}
Conventional and real-time PCR were used to detect transgenic DNA in digesta, faeces and blood collected from six ruminally and duodenally cannulated sheep fed forage-based (F) or concentrate-based (C) diets containing 15% Roundup Ready (RR) rapeseed meal (n 3). The sheep were adapted for 14 d to F or C diets containing non-GM rapeseed, then fed the RR diets for 11 d. On day 12, they were switched back to non-GM diets for a further 11 d. Ruminal and duodenal fluids (RF, DF) and faecal samples… CONTINUE READING

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