• Corpus ID: 1326709

Controversial Cases of Human Gender Identification by Amelogenin Test

  title={Controversial Cases of Human Gender Identification by Amelogenin Test},
  author={L. G. Kao and L. C. Tsai and James Chun-I Lee and Hsing-Mei Hsieh},
Gender identification is essential on forensic science to provide the initial information for criminal investigation. However, gender identification by amplification of amelogenin gene sometimes showed controversial cases, in which male samples was falsely identified as females. We reported the anomalous amelogenin results of 8 samples in Taiwan. The frequency was about 0.00625 % for males and 0.015 % for females, and lower than those of the other populations reported. To confirm amelogenin… 

Tables from this paper

Human Identification by Amelogenin Test

Amelogenin testing is a part of many genetic profiling multiplex systems used to determine if the sample being tested is of male or female origin 1. The gender of an offender determined from criminal

Sequence variation of the amelogenin gene on the Y-chromosome

This study determined the size of the Y-chromosome deletion that resulted in the failure of the amelogenin sex test in two black South African AMELY-negative males by typing specific DNA markers surrounding the amELogenin locus and confirmed that these deletions do occur in the African population.

Sex determination problems in forensic genetic analysis

Amel Gene Profiling from Toothbrush for Sex Determination Among Tanzanians in Surabaya, East Java.

Toothbrush as source of biological samples and Amelogenin gene through DNAband contrast analysis is helpful in sex determination among Tanzanians, which contributes to bench marking applicability of Amel gene analysis as a successful and reliable method of sex determination to localized population.

Forensic Science International: Genetics Supplement Series

Different cases related to sex determination associated with mutations in the amelogenin sex marker or in theirs primers annealing regions, hermaphroditism, aneuploidies and the potential results of the forensic genetic routine markers are proposed whose interpretation should be used carefully to avoid misunderstanding in reports.

Nepalese Null AMELY Males and their Y-Haplotypes

No amplification of DYS458 Marker in all null AMELY males but great sharing of amplified Y STR alleles among them is a probable sign of the general phylogenetic origin.

Isolation of epithelial cells from tooth brush and gender identification by amplification of SRY gene

Introduction: This study determines the importance of tooth brush from which DNA can be isolated and used for sex determination in forensic analysis. Materials and Methods: A total of 30 samples were

Isolation of epithelial cells from acrylic removable dentures and gender identification by amplification of SRY gene using real time PCR

It is concluded that saliva-stained acrylic dentures can act as a source of forensic DNA and co-amplification of SRY gene with other routine sex typing markers will give unambiguous gender identification.

Practical aspects of DNA-based forensic studies in dentistry

A description of the various sources in the oral region has been provided from which samples could be forwarded to the forensic laboratory, and most commonly employed techniques of collection and handling for laboratory procedures have been outlined.

DNA phenotyping and drug induce pigmentation: a theoretical approach

Drug influence in pigmentation is an area with almost no studies, having only a few that mention pigmentation but do not explore this any further mainly because the ones who focus on drugs effects on organisms normally are more related to pathologies being the pigmentation a characteristic that is easily discarded.



Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis?

This study showed a deletion of Y chromosome-specific amelogenin in five Indian males, who are proposed to call “deleted-amelogenin males” (DAMs), who but for the detection of the presence of other Y-specific markers would have been identified as females.

Rare failures in the amelogenin sex test

Among 29, 432 phenotypic male individuals stored in the Austrian National DNA database, 6 individuals were found to lack the amelogenin Y-specific PCR product which was confirmed using alternative amelogensin primers.

Higher failures of amelogenin sex test in an Indian population group.

In this study of 338 male individuals in a Malaysian population, using the AmpFlSTR Profiler Plus kit, the amelogenin test gave a significant proportion of null alleles in the Indian ethnic group and 0.88% frequency in the Malay ethnic group due to a deletion of the gene on the Y chromosome.

An investigation of sequence deletions of amelogenin (AMELY), a Y-chromosome locus commonly used for gender determination

Comparisons of both deletion size and haplotypes with published data suggest that most AMELY nulls are the result of independent evolutionary events, even in those populations where the frequency is relatively high.

Male amelogenin dropouts: phylogenetic context, origins and implications.

A rare mutation in the primer binding region of the amelogenin gene can interfere with gender identification.

Reliability of DNA-based sex tests